Talk:Gene knockout

Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Alv3307.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 22:09, 16 January 2022 (UTC)

Untitled

References? 128.2.153.48 (talk) 22:06, 25 November 2009 (UTC)

Proposed Merge Between 'Gene knockout' and 'Knockout mice'

I conisider that knockout mice are an application of the concept of a gene knockout and should be included as a section in the Gene knockout article rather than stand as an article in their own right. --Username132 (talk) 11:34, 23 August 2006 (UTC)

• I disagree, the mouse is specific and a widely used technilogy. While this article is about the technology the mouse article offers the possibility of providing information of mouse modles for disease.--Peta 00:05, 25 September 2006 (UTC)

Both versions work, but to mee it seems logical letting the mice have their own article. // habj 15:02, 2 October 2006 (UTC)

• Oppose The mice is a product of the technology, and not part of the gene knockout technology itself. What is needed on this article is merge the section on knocking out technique to the gene knockout article and expand with details of the mice. XIZIX 10:49, 31 October 2006 (UTC)

Someone's Unlabelled Text

I stand corrected.

I mixed two concepts, only to be corrected immediately by my girlfriend whom I asked to check it.

Part of this text may be reused elsewhere later.

Often knockout genes are produced as follows:

• First random mutations are introduced in the DNA sequence of the species under investigation, by chemical or other means.
• Offspring from the treated individuals are scanned for a non characteristic trait that is of interest to the investigator. This is typically a lengthy process since only one or a few individuals in a population of many thousands will by chance have the required anomaly.
• Thus selected individuals are used to breed a population in which the trait is omnipresent. This may take several generations of selective breeding. Therefore fast growing plants or animals are often preferred, when the process under investigation is not specific for a certain species. Fruit flies are a typical example, since a new generation can be bred in a matter of weeks.
• The new slightly changed individual can now be further examined to find all causal relations that are related to the introduced anomaly. The investigation may reveal:
  • Causes that led to the first observed anomaly.
    A researcher may start to select e.g. fruit flies with an abnormal wing structure and trace this morphological defect back to the proteins that are missing, overproduced or produced at the wrong moment, then back again to the position in the DNA where the mutation occurred thus discovering (part of) the function of the gene that resides at that location.
  • Effects that follow from the observed anomaly.
    A researcher may start to select mutated individuals that miss a certain known protein in order to find any morphological, behaviouristic or other anomalies, which may then be (partially) explained form the protein deficiency. —Preceding unsigned comment added by Erik Zachte (talk • contribs)

What is to stop people

Once genetic engineering becomes well developped, what is to stop people from doing planned extinctions on each other by inserting recessive knockout genes just like it is advocated for malaria carrying mosquitoes? --Sillybilly 08:26, 30 November 2006 (UTC)

On each other? Lack of technical expertise for one... Gene knockout isn't something you can do to your friends; it's applied when creating new individuals from the embryo stage. --Seans Potato Business 21:49, 1 January 2008 (UTC)

conditional deletion

Is the term "conditional (sequence) deletion" equivalent to gene knock out? Certainly it is less commonly used than gene knockout.

Should there be a redirect sign to this page then?

What do people think? --Wuerzele (talk) 02:01, 20 February 2014 (UTC)

Proposed Major Changes

Hi, I am editing this article as part of a school assignment. I have a few proposed major changes that I will be implementing within the next few weeks unless anyone disagrees with any of the items mentioned below:

1) The entire paragraph beginning with "the directed creation of a KO begins with..." is almost word-for-word plagiarized (without citation), from "Gene Knockout Technology and It's Application" by Deepak Kumar Baranwal, Prakash Singh, Ramesh Kumar Singh,and Shashank Shekhar Solankey. I will remove this section and find another way to explain the concept.

2) There are newer methods for gene knockouts that are not represented here, including zinc fingers, TALENs, and CRISPR. I will add sections on these techniques.

3) The methods section seems unorganized to me. I propose to organize it with subheadings based on the different methods, including the ones I plan to add.

4) I may add to the uses section if I find any newer applications of the technology.

If anyone has any comments on these changes, please let me know. --Alv3307 (talk) 18:38, 27 March 2018 (UTC)

I am deleting these paragraphs because some seem to be very similar to an uncited source, or I think can be more succinctly summarized, but I am saving them here in case anyone wants to reword and reincorporate in place of what I have changed it to:

"The directed creation of a KO begins in the test tube with a plasmid, a bacterial artificial chromosome or other DNA construct, and proceeding to cell culture. Individual cells are genetically transfected with the DNA construct. Often the goal is to create a transgenic animal that has the altered gene. If so, embryonic stem cells are genetically transformed and inserted into early embryos. Resulting animals with the genetic change in their germline cells can then often pass the gene knockout to future generations.

The construct is engineered to recombine with the target gene, which is accomplished by incorporating sequences from the gene itself into the construct. Recombination then occurs in the region of that sequence within the gene, resulting in the insertion of a foreign sequence to disrupt the gene. With its sequence interrupted, the altered gene in most cases will be translated into a nonfunctional protein, if it is translated at all.

Because the desired type of DNA recombination is a rare event in the case of most cells and most constructs, the foreign sequence chosen for insertion usually includes a reporter. This enables easy selection of cells or individuals in which knockout was successful. Sometimes the DNA construct inserts into a chromosome without the desired homologous recombination with the target gene. To eliminate such cells, the DNA construct often contains a second region of DNA that allows such cells to be identified and discarded."

I am also removing this part on moss, because it doesn't seem to fit anywhere in the existing article. If a section was created on more species specific knockouts, or someone thinks there is a good place for it, this could maybe be added back in:

"To create knockout moss, transfection of protoplasts is the preferred method. Such transformed Physcomitrella-protoplasts directly regenerate into fertile moss plants. Eight weeks after transfection, the plants can be screened for gene targeting via PCR." Reski R (June 1998). "Physcomitrella and Arabidopsis: the David and Goliath of reverse genetics". Trends in Plant Science. 3 (6): 209–210. doi:10.1016/S1360-1385(98)01257-6. --Alv3307 (talk) 14:13, 10 April 2018 (UTC)

Recent Development In Biotechnology

Hi, my name is Yasin. I'm a Molecular Biology master student and editing the page was given to me as an assignment for Recent Developments in Biotechnology (MLC501) in Uskudar University. Yasinay689 (talk) 09:56, 26 January 2023 (UTC)